Black Truffle

Iranian Black Truffle

The Truffles were studied and collected from two towns of Firuzabad and Sarvestan in Fars Province, southern Iran. They were analyzed in terms of chemical properties (carbohydrate, protein, reducing sugar, antioxidant, fat, minerals, and ash) according to the Association of Official Analytical Chemists (AOAC) procedures. 

The sequence alignment and tree were determined using Molecular Evolutionary Genetics Analysis (MEGA7) software. The Internal Transcribed Spacer (ITS) region of rDNA of the two truffles was amplified using ITS1 and ITS4 primers and were sequenced. The phylogenic analysis was conducted using Nucleotide Basic Local Alignment Search Tool (BLAST) in the GeneBank (NCBI).

Results: 

The samples contained 63.5 and 66% carbohydrate, 13.06 and 12.93% protein, 5.81 and 5.69% fat, 5.16 and 5.05% ash, 2.05 and 1.72% reducing sugar based on dry weight, respectively. The truffles belonged to the species of Terfezia claveryi and the IST sequences of the truffles of the two areas were similar.